Anti-β-actin antibody

Anti-β-Actin Antibody

Overview

Cat. Number: BA1104

Product Name: Anti-β-Actin antibody

Reactivity: Human, Rat, Mouse, Pig, Chicken, Monkey

Clone Number: 6G3

Host species: Mouse

Clonality: Monoclonal

Isotype: IgG1

Immunogen: Full-length β-Actin protein of human

Applications

Tested applications: WB, IHC. Not yet tested in other applications.

Recommended dilutions: 

WB: 1:5000-1:20000

IHC: 1:200

Optimal dilutions/concentrations should be determined by the end user.

Properties

Form: Liquid

Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.

Storage: β-Actin antibody is stable for 1 month at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Background

β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.

For Research Use Only.