Anti-CD68 antibody

Overview

Cat.Number: BA1023

Product Name: Anti-CD68 antibody

Reactivity: Reacts with Human; Predicted to work with: Monkey

Clone Number: C68/684

Host species: Mouse

Clonality: Monoclonal

Isotype: IgG1

Immunogen: Recombinant full length protein corresponding to Human CD68. (Subcellular fraction of Human alveolar macrophages).

Applications

Tested applications: Flow Cyt,WB,IHC-P.ICC/IF. Not yet tested in other applications.

Recommended dilutions:

WB: Use a concentration of 1 - 2 µg/ml.

Flow cyt: Use 1-2µg for 106 cells.

IHC-P：Use a concentration of 1 - 2 µg/ml.

ICC/IF: Use a concentration of 1 - 2 µg/ml.

Optimal dilutions/concentrations should be determined by the end user.

Properties

Form: Liquid

Concentration:100 µl at 1 mg/ml

Storage: Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

Cellular localization：Cell membrane and Endosome membrane. Lysosome membrane.

Positive control

IHC-P: Human tonsil and histiocytoma tissues;

ICC/IF: U-87 MG cells;

Flow Cyt: U-87 MG cells;

WB: Human spleen tissue lysate.

Background

ab201340 stains macrophages in a wide variety of Human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

For Research Use Only.